Paper chromatography of free amino acids in human blood serum.

BOTJLANGER and BISERrE (1) used a solution of acid acetone for the extraction of amino acids in biologic material. The separation of amino acids on buffered papers described by Ryb#{225}r, Touek, and Hais, (2) and Hais and Hordovs/q (3) used the combination of tertiary butanolborate buffer system (pH 8.5) for separation of amino acids on paper. In our laboratories we have developed a chromatographic method for the separation of free amino acids in human blood serum in which previous desalting is not necessary. One milliliter of blood serum is deproteinized with 9 ml. of ethanolacetone (7:3 [V/V]). After centrifugation the whole supernatant is